Degradation of cellulose is brought by some bacterial species with the help of the enzyme cellulase. Chemically it is polysaccharide of glucose residues with beta 1, 4 glycosidic linkage. Observe the plates for starch hydrolysis around the streaking of each organism and check the color change of the medium,ĭiscoloration of iodine around colonies indicates hydrolysis of starch by amylase and vice-Īim: To perform cellulase production testĬellulose is a basic structural material of plant species. Flood the plate with grams iodine solution with a dropper for 30 seconds and drain off excess iodine.Incubate the plate at 37☌ for 48 h ( bacteria) or at 25☌ for 4-5 days ( fungi). ![]() Inoculate the starch plate with the organism (by single streak) under aseptic condition.Prepare the sterile starch agar plate and label it with the name of organism to be inoculated.Apparatus: Petri dish, nichrome wire loop, dropper, glass marking pencil.Bacterial culture: Nutrient broth consist of E.coli and Bacillus subtilis.If a microorganism is able to produce enzyme amylase, it hydrolyzes starch into maltose and glucose which indicates by the disappearance of color around colonies. In this test, iodine reagent is added into media which gives bluish-black color by the formation of a complex with starch. This test is based on the principle that amylase results in the breaking of starch in sugars. Starch is a complex carbohydrate made up of glucose which acts as source of carbon and energy. It hydrolyzes starch into sugars by breaking down glycosidic linkage. Citrate Utilization Test Amylase detection testĪim: To perform amylase detection test Introduction:Īmylase is an enzyme present in all living organism which plays important role in chemical process of digestion. By using Kovacs reagent or Ehrlichs reagentġ3.Mannitol fermentation test by using “Mannitol salt agar media’.Mannitol fermentation test by using ‘Phenol red Mannitol broth’. ![]() Fermentation of sugars like Glucose, lactose, or sucrose.It includes various tests as given below ![]() Step II: Biochemical test for final confirmation.Įxamination of microorganisms under microscopical examination, biochemical test plays important role in identification, classification, and confirmation of microbes. Identification of microorganisms is done in two steps By using Kovacs reagent or Ehrlichs reagent
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